FIGURE 1.

Overexpression of definite human LRP1 fragments for functional assays. A, schematic representation of LRP1 domain structure and fragments cloned for functional analysis of interactions with AgLDL. These fragments were expressed and purified from CHO-K1 cell supernatants. (See “Experimental Procedures” for details.) *, NPXY motifs. B, Western blot analysis of Ni-NTA-purified recombinant proteins (LRP1 clusters II, III, and IV as well as cluster II fragment CR4–CR8) detected using anti-Myc antibody.