FIGURE 4.

Demonstration of direct physical interactions between the C-terminal half of domain CR9 and AgLDL. A, schematic representation of the receptor-ligand binding fluorometric assay used to study interactions between immobilized BSA-conjugated P1, P2, and P3 peptides and AgLDL. (See “Experimental Procedures” for details.) B, quantitation of peptide-bound cholesterol according to the results of the fluorometric assay. C, effect of increasing concentrations of anti-P3 Abs on P3-bound cholesterol. Results are shown as mean ± S.D. (error bars) of six different experiments performed in duplicate. *, p < 0.05 versus no Ab.