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The enhancing effect of DTT on PRMT1 methyltransferase activity is independent of the His6 tag. Enzymatic activity of HisTevPRMT1 (○), cleaved PRMT1 (▵), and dialyzed HisTevPRMT1 (□) measured with 200 μm R3 peptide in the absence (open symbols) and presence (closed symbols) of DTT, respectively, as well as HisTevPRMT1 treated with EDTA only as a control (♢).
