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. 2015 Apr 15;290(24):15030–15041. doi: 10.1074/jbc.M114.632794

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Rapid recruitment of Elongin A and CUL5 after microirradiation of living cells. A, localization of Halo-Elongin A and mCherry-CUL5 in U2OS cells. B, Hoechst-sensitized U2OS cells expressing Halo-Elongin A and mCherry-CUL5 were microirradiated and imaged 1 s before (−1 s) or at the times indicated after initiation of microirradiation. C, unsensitized U2OS cells expressing Halo-Elongin A and mCherry-CUL5 were microirradiated along a stripe running between the white arrows and imaged as in B.