FIGURE 6.

A possible mechanism underlying the GFP folding reaction mediated by the ELΔC₁₄·ES₇ bullet-shaped complex (A) and by the ELΔC₁₄·ES₁₄ football-shaped complex (B). A, in the case of the single ring variant SR1ΔC₇·ES₇ and bullet-shaped complex ELΔC₁₄·ES₇, the truncation of 23 residues in the C terminus resulted in the escape of the GFP molecule in its denatured conformation. Once the folding reaction of the acid-denatured GFP was completed within the GroEL·ES complex, the molecule did not escape from the chamber. B, in the case of the football-shaped ELΔC₁₄·ES₁₄ complex, escape from the chamber was prevented by GroES on both rings. However, translocation between the two rings may occur before the completion of refolding within the chamber.