FIGURE 6.

Effects of Triton X-100, S-adenosylmethionine, and pH on OlsG activity. A, OlsG-dependent OL consumption was determined at different time points under standard conditions over 60 min. B, OlsG methyltransferase activity was assayed at varying Triton X-100 concentrations. C, OlsG activity was assayed at varying SAM concentrations. The best fit (the nonlinear regression curve fitted to the Michaelis-Menten equation) is shown as a solid gray line. D, OlsG methyltransferase activity was assayed at the indicated pH values with 50 mm BisTris/HCl (□) or 50 mm Tris/HCl (■). Reaction time in B–D was 60 min. The values in A–D are shown as means ± S.D. (n = 3).