FIGURE 6.

Sequence alignment between the RH domains of RH-RhoGEF protein family and Rgnef. Amino acids are colored based on conservation according to the Clustal scheme. Gold boxes over the alignment indicate the localization of helices based on the crystal structures. Numbers indicate the start and end of the RH-like domain in the respective sequence as follows: p115(h):Q92888, Lsc(m):Q61210, Lsc(r)Q9Z1I6, PDZ(h):O15085, PDZ(r):Q9ES67, LARG(h):Q9NZN5, LARG(m):Q8R4H2, Rgnef(h): Q8N1W1, Rgnef(m):P97433, Rgnef(r):P0C6P5. Five conserved Rgnef human (h) residues subjected to alanine mutagenesis are boxed. m, mouse.