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Anti-tyrosinase and copper chelating effects of Efse-EA. Tyrosinase was preincubated with the test substances at 25 °C for 5 min prior to incubation with l-tyrosine for 30 min; absorbance was read at 490 nm. Each determination was made in triplicate and the data shown represent the mean ± SD. ** p < 0.01, Student’s t-test (A); copper chelating effects were measured using pyrocatechol violet (PV) as described in Experimental Section (B).
