Figure 3.
Effect of Efse-EA on melanogenesis in melan-a cells. Cells (1 × 105 cells/mL) were seeded for overnight, and then the medium was replaced with fresh medium containing the indicated concentrations of Efse-EA or PTU (N-phenylthiourea). Cells were cultured for 72 h and further incubated for 24 h. After washing with PBS, the cells were lysed with 250 μL of 1 N NaOH and transferred to a 96 well plate. The melanin contents were estimated by measuring the absorbance at 405 nm. Each determination was made in triplicate and the data shown represent the mean ± SD. * p < 0.05, ** p < 0.01, Student’s t-test.