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Effect of Efse-EA on intracellular tyrosinase activity. Melan-a cells (1 × 105 cells/mL) were cultured for 24 h; the medium was replaced with fresh medium containing the indicated concentrations of Efse-EA or arbutin for 3 days. The cells were collected and lysed. After quantifying protein levels, tyrosinase activity was determined by l-DOPA zymography. Each determination was made in triplicate and the data shown represent the mean ± SD. ** p < 0.01, Student’s t-test.
