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. 2015 Apr 23;16(5):9217–9235. doi: 10.3390/ijms16059217

Table 1.

Anti-oxidant activity of E. ferox seed ethyl acetate fraction (Efse-EA) in various in vitro assay systems.

Compound Concentration (µg/mL) DPPH (1) FRAP (2) CUPRAC (3) ABTS (4)
Activity (% of Control)
Ascorbic acid 1 4.5 ± 0.011 (5) 12.7 ± 0.002 ** 12.2 ± 0.001 −1.7 ± 0.002
3 8.5 ± 0.016 **,(6) 52.5 ± 0.001 ** 42.7 ± 0.001 ** 2.7 ± 0.001 **
10 18.5 ± 0.054 * 170.8 ± 0.006 ** 147.8 ± 0.001 ** 18.1 ± 0.003 **
30 57.3 ± 0.069 ** 523.3 ± 0.026 ** 119.2 ± 0.002 ** 57.9 ± 0.008 **
Efse-EA 1 0.6 ± 0.002 19.9 ± 0.003 ** 12.8 ± 0.003 * 5.2 ± 0.001 **
3 7.9 ± 0.012 82.0 ± 0.009 ** 54.3 ± 0.009 ** 18.6 ± 0.013 *
10 28.1 ± 0.006 ** 234.4 ± 0.027 ** 251.9 ± 0.029 ** 55.1 ± 0.024 *
30 78.0 ± 0.021 ** 820.4 ± 0.065 ** 669.6 ± 0.043 ** 89.7 ± 0.001 *

(1) 1,1-Diphenyl-2-picrylhydrazyl radical scavenging assay; (2) Ferric reducing antioxidant power assay; (3) Cupric(II) ion reducing antioxidant capacity assay; (4) 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical scavenging activity assay; (5) Values are expressed as mean ± SD of triplicate determinations; (6) Data was considered to indicate statistical significance (* p < 0.05, ** p < 0.01) by means of the student t-test).