Figure 5.

Comparison of biocompatibility of SEON^LA–BSA directly after synthesis (green bars), and after four weeks of storage at 4 °C (blue bars), 20 °C (yellow bars), 37 °C (black bars) and 45 °C (red bars). (A) Increase of the relative side scatter (SSC) in viable cells after 24 and 48 h of incubation. The relative SSC is given as relative to the respective untreated controls; (B) Percentage of cells which were negative for both Annexin A5-Fluorescein isothiocyanate (Ax) and propidium iodide (PI) staining after 24 and 48 h of incubation; (C) Percentage of DiIC₁ positive cells after 48 h of incubation; (D) Percentage of cells with sub-G1 DNA after 48 h of incubation. All experiments were performed in triplicates. The asterisks mark significance levels that were determined using an unpaired t-test (* p < 0.05; ** p < 0.005; *** p < 0.0005).