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. 2015 Apr 30;16(5):9896–9909. doi: 10.3390/ijms16059896

Figure 1.

Figure 1

Effects of MBP on NO production and cytokine secretion in RAW264.7 macrophage cells. (A) RAW264.7 cells were treated with MBP (0.1–10 μg/mL) or LPS (5 μg/mL) for 48 h; (B) RAW264.7 cells were treated with 5 μg/mL MBP for 12–72 h. NO production was measured by the Griess reaction; (C) RAW264.7 cells were treated with 5 μg/mL MBP or left untreated. The culture supernatants were collected after 24, 48, or 72 h and examined by ELISA for cytokine secretion. Results were presented as mean ± SD of three independent experiments, each performed in triplicate. * p < 0.05 compared with the untreated control.