Figure 3.

Effects of MBP on expression of MHC class I, MHC class II, CD80 and iNOS in RAW264.7 macrophages. RAW264.7 cells were treated with 5 μg/mL of MBP or 1 μg/mL of LPS for 48 h in the presence or absence of 5 μg/mL of polymyxin B (PB). Expression of MHC class I, class II, CD80 and iNOS was analyzed by flow cytometry using specific antibodies and isotype controls. Percentages of cells expressing each of these molecules were calculated. Representative flow plots are shown, and results from five independent experiments are presented as mean ± SD.