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. 2015 Apr 30;16(5):9976–9997. doi: 10.3390/ijms16059976

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Effects of SQE, p-coumaric acid, and tricin on mRNA levels of CD133, CD44, DLK1, Notch1, Sox-2, and VEGF in colon CSCs. CD133⁺CD44⁺ HT29 cells (A) and CD133⁺CD44⁺ HCT116 cells (B) were treated with SQE (0, 100, 200, or 300 μg/mL), or p-coumaric acid (1.8 μM) and tricin (0.7 μM) at concentrations equivalent to that contained in 300 μg/mL SQE. Levels of mRNA for (a) CD133; (b) CD44; (c) DLK1; (d) Notch1; (e) Sox-2; and (f) VEGF were examined using real-time PCR, with detection of β-actin used as an internal control. The letter labels on the histogram indicate the values that significantly differed from each other (p < 0.05) according to one-way ANOVA for multiple comparisons. Ctrl, Control; SQE, Sasa quelpaertensis extract; P.C, p-coumaric acid; T, tricin.