T-cell factor 4 (TCF4) is a potential molecular target of phytochemicals in HCT116 cells. (A) Normal human colon cells and different types of human colorectal cancer cells (HCT116, SW480, LoVo and Caco-2) were lysed, and Western blot was performed for TCF4, β-catenin and actin, as described in the Experimental Section; (B) Normal human colon cells and different types of human colorectal cancer cells (HCT116, SW480, HT-29, LoVo and Caco-2) were grown overnight. Total RNA was extracted, and RT-PCR was performed using primers for TCF4 and GAPDH; (C) HCT116 cells were treated with 50 µM of epigallocatechin gallate (EGCG), resveratrol, genistein and capsaicin for 24 h, and a Western blot was performed for TCF4, β-catenin and actin.