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. 2015 May 7;16(5):10411–10425. doi: 10.3390/ijms160510411

Figure 4.

Figure 4

Resveratrol increase phosphorylation of TCF4 in serine/threonine residues through ERK (extracellular signal-regulated kinase) and p38 MAPK (mitogen-activated protein kinase)-dependent pathway. (A) HCT116 cells were treated with DMSO or resveratrol for 6 h, and the cell lysates were immunoprecipitated with TCF4 antibody and then immunoblotted with serine/threonine-specific antibody; (B) HCT116 cells were pre-treated with selective inhibitors for ERK (PD98059), p38 MAPK (SB203580) and NF-κB (BAY11-7082) for 2 h and then co-treated with resveratrol for 6 h, and Western blot was performed for antibodies against TCF4 and actin; (C) HCT116 cells were treated resveratrol (upper) or DMSO (lower) for indicated time points, and then, Western blot was performed for phospho-ERK, ERK, phospho-p38 and p38; (D) HCT116 cells were pre-treated with selective inhibitors for ERK (PD98059) and p38 MAPK (SB203580) for 2 h and then co-treated with resveratrol for 6 h, and the cell lysates were immunoprecipitated with TCF4 antibody and then immunoblotted with serine/threonine-specific antibody.