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. 2015 May 14;16(5):10934–10951. doi: 10.3390/ijms160510934

Table 1.

RT-PCR and RT-qPCR primers sequences specific to porcine MSCs and differentiated cells.

Gene Primer Sequence (5'–3') Products Sizes (bp) Annealing Tm (°C) Reference/Accession Number
OCT4 F-AGGTGTTCAGCCAAACGACC 335 60 Carlin et al., 2006
R-TGATCGTTTGCCCTTCTGGC
SOX2 F-GCCTGGGCGCCGAGTGGA 443 64 Carlin et al., 2006
R-GGGCGAGCCGTTCATGTAGGTCTG
NANOG F-ATCCAGCTTGTCCCCAAAG 438 60 Carlin et al., 2006
R-ATTTCATTCGCTGGTTCTGG
CD105 F-CGCTTCAGCTTCCTCCTCCG 281 56 Miernik & Karasinski, 2012
R-CACCACGGGCTCCCGCTTG
CD140b F-TACGTGCCCATGCTGGACATG 175 54 Miernik & Karasinski, 2012
R-TGGTAGCTGAAGCCCACGAG
CD144 F-TGCAACGAGCGGGGCGAGTT 220 56 Miernik & Karasinski, 2012
R-CGCCGCCCTCCTCATCGTA
FABP F-TGGTACAGGTGCAGAAGTGGGA 100 60 NM_001002817
R-GCCGTGACACCTTTCATGATACA
LPL F-CAAACTTGTGGCTGCCCTAT 202 60 Kumar et al., 2007
R-AAGGCTGTATCCCAGGAGGT
AP2 F-AACCCAACCTGATCACTG 192 60 AF102872.1
R-TCTTTCCATCCCACTTCTGC
ON F-TCCGGATCTTTCCTTTGGTTTCTA 187 60 Kumar et al., 2007
R-CCTTCACATCGTGGCAAGAGTTTG
RUNX2 F-CAGACCAGCAGCACTCCATA 167 60 XM_003482203
R-AACGCCATCATTCTGGTTAG
BG F-GTCGTCCAGTGCTCTGACCT 93 60 XM_003135475
R-GGAGCTCGGAGATGTCGTTA
B3T F-CAGAGCAAGAACAGCAGCAGCTACTT 227 60 XM_003480812
R-GTGAACTCCATCTCATCCATGCCCTC
NFM F-GTCAGACCAGGCAGAAGAGG 222 60 Kumar et al., 2012
R-GATTTGGGCATAGGGGATTT
NES F-AGGAACCAAAAGAGGCAGGT 229 60 Kumar et al., 2012
R-TTGGGACCAGGGACTGTTAG
NGF F-CACACCGAGAGCAATGTCCC 130 60 XM_005674272
R-CCACCCTGGCGGCTATCGCC
MBP F-GAGATGGCTCAACTCAGAACG 125 60 NM_001001546
R-GGTTAGTATTTGCCGTGAGCA
MAP2 F-GCCATTATTCGTACACCTCCA 291 60 XM_005672149
R-AGAGCCGCATTTGGATGTCAC
HMBS F-TTCATTCCCTCAAGGACCTG 101 60 NM_001097412
R-GGGGTGAAAGACAACAGCAT