Figure 3.

DOX impaired platelet function. (A,B) PRP or washed platelets were incubated with different concentrations of DOX or solvent. Platelet aggregation was induced by addition of ADP (A) or thrombin (B); representative traces from three independent experiments are shown; (C) Platelets were incubated with different concentrations of DOX or solvent. Treated platelets were perfused into vWF-coated glass capillary. The results from three independent experiments are shown as the means ± SEM of cell number/mm². * p < 0.017 (after Bonferroni correction) as compared with solvent; (D) Platelets were incubated with different concentrations of DOX or solvent. Treated platelets were centrifuged, and the supernatants were analyzed by Western blot with SZ-2. Representative data of three independent experiments are presented.