Table 2.
Primary antibodies used for immunohistochemical staining of aortic tissue samples.
| Gene Symbol * | Gene ID * | Protein Symbols | Full Name | Catalog Number | Supplier | Species |
|---|---|---|---|---|---|---|
| PTK2B | 2185 | PTK2B, PYK2, PKB, PTK | Protein tyrosine kinase 2 β | ab55358 ¶ | Abcam, Cambridge, MA, USA | Rabbit polyclonal |
| LAT | 27040 | LAT, pp36 | Linker for activation of T cells | ab32070 § | Abcam, Cambridge, MA, USA | Rabbit monoclonal |
| VAV3 | 10451 | VAV3 | Vav 3 guanine nucleotide exchange factor | ab40889 | Abcam, Cambridge, MA, USA | Rabbit polyclonal |
| RAC2 | 5880 | RAC2, HSPC022, p21-Rac2, EN-7 | Ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2) | ab2244 † | Abcam, Cambridge, MA, USA | Goat polyclonal |
| TNF | 7124 | TNF, TNFA, TNFSF2, DIF | Tumor necrosis factor | ab6671 | Abcam, Cambridge, MA, USA | Rabbit polyclonal |
| CD244 | 51744 | CD244, 2B4, NAIL, NKR2B4, SLAMF4 | CD244 molecule, natural killer cell receptor 2B4 | HPA010628 † | Sigma-Aldrich, St. Louis, MO, USA | Rabbit polyclonal |
| TYROBP | 7305 | DAP12, KARAP, PLOSL | TYRO protein tyrosine kinase binding protein | sc-20783 ¶ | Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA | Rabbit polyclonal |
| HCST | 10870 | DAP10, KAP10, PIK3AP | Hematopoietic cell signal transducer | sc-10531 ¶ | Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA | Goat polyclonal |
| SH2D1A | 4068 | SAP, LYP, DSHP, MTCP1, EBVS | SH2 domain containing 1A | sc-8333 † | Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA | Rabbit polyclonal |
| VAV1 | 7409 | VAV1, VAV | Vav 1 guanine nucleotide exchange factor | sc-132 | Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA | Rabbit polyclonal |
| PLCG1 | 5335 | PLCG1, PLC1, NCKAP3, PLC148 | Phospholipase C, gamma 1 | sc-7290 | Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA | Mouse monoclonal |
| PLCG2 | 5336 | PLCG2, FCAS3, APLAID | Phospholipase C, gamma 2 (phosphatidylinositol-specific) | sc-5283 ¶ | Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA | Mouse monoclonal |
| RAC1 | 5879 | RAC1, MIG5, TC-25, p21-Rac1 | Ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) | 05-389 † | Millipore Corporation, Billerica, MA, USA | Mouse monoclonal |
| RAF1 | 5894 | RAF1, NS5, CRAF | v-raf-1 murine leukemia viral oncogene homolog 1 | sc-7267 † | Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA | Mouse monoclonal |
| GZMB | 3002 | GZMB, HLP, CCPI, CGL1, SECT, CTLA1, CTSGL1 | Granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) | sc-1969 ¶ | Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA | Goat polyclonal |
| CD8A | 925 | CD8, MAL, p32, Leu2 | CD8a molecule | M7103 ¶ | Dako, Glostrup, Denmark | Mouse monoclonal |
| CD68 | 968 | CD68, GP110, LAMP4, SCARD1 | CD68 molecule | M0876 ¶ | Dako, Glostrup, Denmark | Mouse monoclonal |
* HGNC approved gene and IDs are available from the HUGO Gene Nomenclature Committee [48] or National Center for Biotechnology Information [45]; † Antibody did not stain positive controls and was not used for staining of aortic samples; § Antibody stained positive controls but did not stain aortic tissue samples and results are, therefore, not reported here; ¶ Used for double-staining.