Table 3.
qPCR primers used to validate RNA-seq data.
| Gene Name | Reference Gene | Annotation | Primer (5'–3') |
|---|---|---|---|
| XLOC_023615 | Capana06g000792 | SWEET sugar transporter | F: ATTGCTCCAAAGCCACCACC |
| R: TGGCAGCATCGTCTCGTTCA | |||
| XLOC_004633 | Capana01g001100 | Major intrinsic protein, conserved site | F: TTGTGGCTGTTTCAGTGTCA |
| R: GGTAGCAATCTTGAGGAGGA | |||
| XLOC_021386 | Capana05g000172 | Proteinase inhibitor I25, cystatin, conserved region | F: AGGCGAAGACAAATCTGGAAT |
| R: TGCTAAATAGTTATGTGGCGAGTC | |||
| XLOC_021757 | Capana05g001613 | Plant peroxidase | F: GTATTACTCGGCAGAAGGGACTC |
| R: GTGGTTGGGCTTGTGGTGT | |||
| XLOC_021200 | Capana05g002178 | Plant peroxidase | F: CTTTTCCACGATTGTTTTGTTAGG |
| R: CGACCTGCTGGCACTGAAT | |||
| XLOC_021142 | Capana05g001951 | AP2/ERF domain | F: TCCTCATACCTAAACGAACCCA |
| R: AGTTGTTGTCGTGTGTTGGATTG | |||
| XLOC_021821 | Capana05g001948 | AP2/ERF domain | F: TTGAAAGAATCTCGGACACCC |
| R: GAAATTGAACGGCGACCAG |