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Effect of miR-24 gain or loss on the mRNA expression of osteogenesis-related genes. RT-qPCR was performed to detect the mRNA expression level of ALP (A), ColA1 (B), OPN (C) and Runx2 (D) in BMSCs and MC3T3-E1cells. Cells transfected with miR-24 or anti-miR-24 (day 0) for 48 h and then cultured in differentiation medium for induction of osteogenic differentiation. Cells were harvested at day 7 for analysis. N = 3, ** p < 0.01 vs. miR-NC; and && p < 0.01 vs. anti-miR-NC.
