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. 2015 May 21;16(5):11699–11712. doi: 10.3390/ijms160511699

Figure 4.

Figure 4

miR-24 directly targets the 3'-UTR of Tcf-1. (A) The 3'-UTR of Tcf-1 had the putative binding sites with miR-24; (B) The direct binding relationship between 3'-UTR of Tcf-1 and miR-24 was detected by a dual luciferase activity assay. The wild type or mutated 3'-UTR of Tcf-1containing the putative binding sites for miR-24 in pGL3 luciferase reporters was transfected into MC3T3-E1cells cells with miR-24 or anti-miR-24. After 48 h of incubation, cells were harvested and luciferase activity was measured by the dual luciferase reporter assay system. N = 6, * p < 0.05 vs. miR-NC; and & p < 0.05 vs. anti-miR-NC.