Figure 6.

Tcf-1 knockdown abrogated the effect of anti-miR-24 on osteoblast differentiation. (A) Western blot analysis of Tcf-1 protein expression in anti-miR-24 transfected MC3T3-E1 cells in the presence with Tcf-1 siRNA. Anti-miR-NC and siNC were used as the control; (B) RT-qPCR analysis of Runx2 mRNA expression in the different groups; (C) ALP activity was detected by an ALP activity assay in the different groups after seven days of osteogenic differentiation; (D) Mineralization was assessed using Alizarin red S staining at day 7. N = 3, * p < 0.05.