Table 2.
Strains used in this study
| Strain | Genotype | Reference |
|---|---|---|
| BY4741 | (Euroscarf) MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | Euroscarf |
| ∆ysp1 | BY4741 YHR155W::KANMX4 | Euroscarf |
| ∆ysp2 | BY4741 YDR326C::KANMX4 | Euroscarf |
| ∆sip3 | BY4741 YNL257C::KANMX4 | Euroscarf |
| ∆lam4 | BY4741 YHR080C::KANMX4 | Euroscarf |
| ∆lam5 | BY4741 YFL042C::KANMX4 | Euroscarf |
| ∆lam6 | BY4741 YLR072W::KANMX4 | Euroscarf |
| WPY361 (upc2-1) | MATa upc2-1 ura3-1 his3-11,-15 leu2-3,-112 trp1-1 | (Li and Prinz, 2004) |
| Upc2-1 ∆ysp2 | WPY361 (upc2-1) YDR326C::KanMX4 | This study |
| Upc2-1 ∆sip3 | WPY361 (upc2-1) YNL257C::HYGROR | This study |
| RS453C | MATα ade2-1 his3-11,15 ura3-52 leu2-3112 trp1-1 | (Levine and Munro, 2001) |
| ∆ysp1 | RS453C YHR155W::HIS5 S.p. | This study |
| ∆ysp2 | RS453C YDR326C::HIS5 S.p. | This study |
| ∆sip3 | RS453C YNL257C::HIS5 S.p. | This study |
| ∆lam4 | RS453C YHR080C::HIS5 S.p. | This study |
| ∆lam5 | RS453C YFL042C::HIS5 S.p. | This study |
| ∆lam6 | RS453C YLR072W::HIS5 S.p. | This study |
| Tcb2-GFP | RS453C TCB2-EGFP::HIS5 S.p. | This study |
| SEY6210 | MATa leu2-3,-112 ura3-52 his3Δ200 trp1Δ901 lys2-801 suc2-Δ9 GAL | (Manford et al., 2012) |
| ANDY198 (Δtether) * | SEY6210 ist2Δ::HISMX6 scs2Δ::TRP1 scs22Δ::HISMX6 tcb1Δ::KANMX4 tcb2Δ::KANMX46 tcb3Δ::HISMX6 | |
| RSY271 sec18-1† | MATa sec18-1 ura3-52 his4-619 | (Novick et al., 1980) |
| Lnp1-GFP‡ | SFNY 2092, MAT a, ura3-52, leu2-3,112, his3Δ200, LNP1-3xGFP::URA3 | (Chen et al., 2012) |
Kind gifts of strains:.
*
Chris Stefan.
†
Mike Lewis.
‡
Susan Ferro-Novick.