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. 2015 May 11;3(5):e12375. doi: 10.14814/phy2.12375

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© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Representative confocal immunofluorescence images of GLUT4 immunofluorescence in human skeletal muscle fibers in the basal state (A, B), 30 min post glucose ingestion (E, F) and 60 min postglucose ingestion (I, J) from experiment 2. Images A, E, and I show GLUT4 localization in green (scale bars 50 μm). Images B, F, and J show more detailed images of GLUT4 in PM regions (scale bars 10 μm). Images C,G, and K show the PM marker dystrophin in red (scale bars 10 μm). Merged images in D, H, and L demonstrate colocalization of GLUT4 with the plasma membrane marker dystrophin (scale bars 10 μm). Open arrows indicate clusters of GLUT4 at the PM, while filled arrow heads indicate moderate continuous GLUT4 signal at the PM.