Fig 5. Macrophage LRP1 deficiency leads to accumulation of triglyceride-rich lipoproteins in the plasma of LDLR-deficient mice.

Mice were fed either a normal chow (a, left; b, left) or Western diet (a, right; b, right; c,d) for six weeks. Aliquots of serum from fasted mice were assayed for cholesterol (a) or triglyceride (b) (* p = 0.0004, **p = 0.003, n = 19 per group, Student’s t-test comparing LRP1+/+ with macLRP1-/-). (c,d) 100 μl of pooled serum (n = 19/group) from macLRP1-/- and LRP1+/+ mice was fractionated over a Superose 6 FPLC column. Plasma cholesterol (c) and triglyceride (d) levels were quantified. Three independent experiments were performed, each with a unique cohort of mice. Data from one representative experiment is shown.