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. 2015 Mar 10;20(4):583–593. doi: 10.1007/s12192-015-0580-5

Fig. 1.

Fig. 1

Heat pretreatment followed by TLR agonist stimulation inhibits cytokine production. WT- or ANXA1-deficient (−/−) macrophages were incubated at either 37 or 42 °C for 1 h prior to stimulation with (a, b, c) LPS (100 ng/ml) or (d, e, f) CPG ODN1826 (1 μM) or (g) Poly(I:C) (10 μg/ml) for 24 h, and the indicated cytokines were measured using ELISA. **p < 0.01 vs LPS 37 °C control. # p < 0.01 vs WT 42 ºC