Fig. 3.

Endogenous ANXA1 is involved in TNFα production after heat and LPS. a Experimental set up for b where supernatants from WT macrophages incubated at either 37 or 42 °C for 1 h were used to treat WT or ANXA1 ^−/− macrophages. TNFα production was measured by ELISA after 24 h after LPS stimulation. c Experimental set up for d where WT or ANXA1 ^−/− macrophages were stimulated with heat for 1 h and media was replaced. LPS was added for 24 h and TNFα production was measured by ELISA. *p< 0.05, **p < 0.01 vs respective LPS 37 °C control