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ANXA1 regulates TNFα mRNA expression and stability. WT or ANXA1−/− macrophages were incubated at either 37 or 42 °C for 1 h prior to stimulation with LPS (100 ng/ml). a TNFα mRNA expression was measured using qPCR after the indicated time points. (b,c,d) TNFα mRNA stability was analyzed after actinomycin treatment. Half-life times were calculated using the equation –ln (2) / decay constant
