Fig. 5.

JNK activation is enhanced, while MKP1 is inhibited in the absence of ANXA1. WT or ANXA1^−/− macrophages were stimulated with LPS for 30 and 60 min or incubated at either 37 or 42 °C for 1 h prior to stimulation with a media or b LPS (100 ng/ml). Cells were lysed for Western blotting for the respective proteins after 30 and 60 min of LPS. Lane numbers are indicated. Fold change was determined using mean gray values of bands divided by their respective total proteins