Expression levels of the c-fos gene measured by real-time PCR in
Dicentrarchus labrax head kidney cells in the course of the
perfusion trial. c-fos mRNA copy number was normalised as a ratio
to 100 ng total RNA. Cells were sampled after the stabilisation period (0 h), 60 min
after highly unsaturated fatty acids (FA) incubation (EPA, DHA, arachidonic acid
(ARA), linoleic acid (LA) and α-linolenic acid (ALA)), 20 min after
adrenocorticotrophin hormone (ACTH) stimulation, and then sequentially at 40, 60,
110, 160 and 250 min following the ACTH pulse. The means of three replicates in each
sampling point are shown. Bars indicate standard error of the mean. Differences were
determined by one-way ANOVA and each time point was analysed separately. A
post hoc test was applied (Tukey). a,b,c Different
letters indicate significantly different means from controls, for the time point
tested (P < 0·01).