Table 2.
Primers and probes for quantitative real-time PCR
| Gene | Symbol | Nucleotide sequence (5′–3′) | One step Taqman real-time standard curve quality |
|---|---|---|---|
| Forward: CAGCAAAATGCCGCAACAG | R2 0·995 | ||
| c-fos | c-fos | Reverse: TGGACTTCTCATCCTCTAGCTGATC | Efficiency 89·45 % |
| Taqman probe: GAGCTTACAGACACTCTG | |||
| Forward: AGCGGAGAATGGACCTACCT | R2 0·982 | ||
| Steroidogenic acute regulatory protein | StAR | Reverse: GAAGACCCAAATAAGACCAAGTTCAC | Efficiency 90·379 % |
| Taqman probe: ATAGTCATGAAGCCCTGTG | |||
| Forward: CTTCGGCAGTAAAGTGCTTTCTAC | R2 0·993 | ||
| Cytochrome P450 11β | CYP11b | Reverse: GGATTTCTGTCGAATGCTGCG | Efficiency 82·47 % |
| Taqman probe: GCTTGATGAGGTGGCGA | |||
| Fw: GGACATCAGCCAGAACAAGAGA | R2 0·996 | ||
| Heat shock protein 70 | HSP70 | Reverse: GAGAACCCTGTCCTCCAGC | Efficiency 99·276 % |
| Taqman probe: GCTTGTGAGAGGGCCAA | |||
| Forward: GCCTTTTGGCATGTACTCAAACC | R2 0·997 | ||
| Glucocorticoid receptor | GR | Reverse: GAACAGGTATGGAGAGTCGTCC | Efficiency 94·417 % |
| Taqman probe: GTGGTTGGGGAGAGCTG | |||
| Forward: CCAACGACTGGGAGGATCAC | R2 0·998 | ||
| Heat shock protein 90 | HSP90 | Reverse: GAGTTCCGGGCCCTGC | Efficiency 86·23 % |
| Taqman probe: CTGTCAAGCACTTCTCG |