Table 1.

Body composition of mice fed either the standard or high-protein diet

(Mean values and standard deviations)

	C20*		C20 3 months		C35*		C35 3 months		S20*		S20 3 months		S35*		S35 3 months
	Mean	sd	Mean	sd	Mean	sd	Mean	sd	Mean	sd	Mean	sd	Mean	sd	Mean	sd
BMD (g/cm3)	0·033	0·001	0·048	0·003	0·032	0·001	0·049	0·002	0·036	0·004	0·043	0·002	0·032	0·004	0·042	0·002
BMC (g)	0·216	0·014	0·407	0·035	0·197	0·023	0·425	0·043	0·243	0·067	0·367	0·032	0·186	0·039	0·369	0·014
LBM (g)	14·23	0·93	21·78	1·49	12·35	0·60	21·78	3·41	14·10	2·04	22·01	2·15	12·00	1·84	22·64	1·07
Percentage fat	16·14	1·04	24·42	4·57	16·13	1·59	25·13	3·68	19·96	2·86	16·98	1·73	18·60	2·00	16·84	2·95

C20, control mice fed a diet supplying 20 % energy from protein; C35, control mice fed a diet supplying 35 % energy from protein; S20, Berkeley sickle mice fed a diet supplying 20 % energy from protein; S35, Berkeley sickle mice fed diet supplying 35 % energy from protein; BMD, bone mineral density; BMC, bone mineral content; LBM, lean body mass; DXA, dual-energy X-ray absorptiometry.

* Values represent a separate set of mice fed the respective diet for 7 d after which the DXA scan was performed. Values at 3 months represent mean DXA measurements recorded after 3 months of feeding. Comparison of the two values will provide a sense of expected increases in body composition with feeding over 3 months.