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. 2015 Jun 11;5:11315. doi: 10.1038/srep11315

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Copyright © 2015, Macmillan Publishers Limited

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(a) Genomic structure of the Fgf10 locus, which includes the target sequence (underlined) and the protospacer-adjacent motif (PAM) sequence (shown in blue), used in this study. (b) Examples of embryos from the three classes that exhibited different limb phenotypes: no limbs (type I), limb defects (type II, left: hindlimb deficiency, right: truncated fore- and hind-limb), and normal (type III). (c) Graph summarizing the effects of Cas9 and gRNA electroporation on limb development. The concentrations of RNAs used in each experiment are shown at left. The numbers in each column are the number of embryos exhibiting the phenotype in each category.