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. 2015 Apr 9;12(2):1868–1876. doi: 10.3892/mmr.2015.3604

Figure 5.

Figure 5

PARP-1 or AMPK inhibition attenuates the proliferation of CNE-2 cells following 10 Gy IR, as determined using an MTT assay. (A) The PARP-1 gene was silenced by RNAi using lentivirus-delivered small-hairpin RNA transfection. (B) Cells were treated with 10 µM Compound C, an AMPK inhibitor, for 24 h. An MTT assay was then used to determine cell proliferation rates in the presence or absence of 10 Gy IR following incubation for 0, 1, 2, 3 and 4 days. Values are presented as the mean ± standard deviation. *P<0.05, vs. CNE-2 and PARP-1 RNAi + 0 Gy IR; #P<0.05, vs. CNE-2 + Compound C (10 µM) + 0 Gy IR. IR, ionizing radiation; PARP-1, poly-(adenosine diphosphate-ribose) polymerase-1; Compound C, 6-[4-(2-Piperidin-1-yl-ethoxy)-phenyl)]-3-pyridin-4-yl-pyrrazolo [1,5-a]-pyrimidine; AMPK, adenosine monophosphate-activated protein kinase; OD, optical density.