Fig. 3.

Representative electron micrographs and characteristics of mitochondrial morphology. Representative electron micrographs from hearts of mice fed normal (A), high-fat diet (B) and high-fat/normal diet (C). Individual mitochondrion area (D) and length (E), mitochondrial coverage of myofilament area (F) and number of lipid droplets per unit area (G) assessed using transmission electron micrographs. Data are presented as mean ± SEM (n = 4 hearts and ≥ 900 mitochondria per heart from ≥ 10 electron micrographs per heart). HF/N = high-fat/normal diet. Data were analysed using Kruskal-Wallis test with Dunn's post-hoc test (D-E) and one-way ANOVA with Bonferroni's post-hoc test (F-G). *** = P < 0.001 and * = P < 0.05 vs. normal diet and ^### = P < 0.001 and ^# = P < 0.05 vs. high-fat diet. The data for mitochondrial area, length and coverage for normal and high-fat diets were taken from [1].