Fig. 5.

Representative Ca²⁺ transient trace and analysis. Data were recorded using Fura-2 AM fluorescence to measure Ca²⁺ transients of cardiomyocytes field stimulated at 0.2, 0.5, 1.0 and 2.0 Hz. A) Representative Ca²⁺ transient trace of cardiomyocytes isolated from mice fed a high-fat/normal diet with the frequencies of stimulation indicated. B) The area under the Ca²⁺ transient. C) The amplitude of the Ca²⁺ transient. D) Time for the Ca²⁺ transient to return to 90 % of the diastolic level. Data are presented as mean ± SEM (n = 22-32 cardiomyocytes from 3-4 hearts). HF/N = high-fat/normal diet and a.u. = arbitrary units. Data were analysed using two-way ANOVA with Bonferroni post-hoc test. *** = P < 0.001, ** = P < 0.01 and * = P < 0.05 vs. normal diet and ^# = P < 0.05 vs. high-fat diet.