Figure 3.

Ions correlated with choline in both marmoset and human samples map to phosphocholine pathways in network enrichment analysis. (A) Venn diagram shows 67 ions were common among the 198 correlated ions for marmosets and 309 correlated ions for humans. (B) Analysis of these 67 ions by MetaCore showed significant pathway enrichments after FDR correction for multiple phosphocholine pathways. (C) Metabolome-wide network structure. Correlation analyses showed that 18 of the 67 ions had similar pairwise correlation patterns for marmosets and humans at p < 0.05. These were tested for correlations among other ions, and features that had absolute Spearman correlation >0.3 at FDR <0.05 are shown for marmosets and humans, demonstrating ability to map the broader network structure associated with choline. Stratification according to strengths of associations showed that the most highly associated clusters contained phospholipids, while terpenoids and steroids were present in less strongly associated clusters.