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. 2015 Jun 11;5:11047. doi: 10.1038/srep11047

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Human mRNAs isolated from peripheral blood mononuclear cells (PBMC) were spiked with viral mRNAs from cell culture infected with human herpesvirus 6B (HHV-6B), human herpesvirus 8 (HHV-8), hepatitis C virus (HCV), Influenza A virus, human Parainfluenza Virus Type 1 (HPIV1), human immunodeficiency virus (HIV), and human Adenovirus Type C. Beta-actin and GAPDH are cellular controls. PATHseq method was performed to enrich the viral sequences. qPCRs were used to monitor the relative enrichments (in fold) of individual virus to cellular gene control, beta-actin. Results are shown from at least three repeats with standard deviation.