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. 2015 Jun 11;5:11066. doi: 10.1038/srep11066

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Copyright © 2015, Macmillan Publishers Limited

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RT-PCR and immunoblotting analyzed the expression of UBE3C in SHG44, U251, U87, TJ861 and TJ899 cells; (B and C) The expression of UBE3C were regulated in TJ899 and U251 cells by RNA interference; (D) Matrigel invasion assays showed that down-regulation of UBE3C was accompanied by a descend invasion of gliomas cells(bar = 100μm); (E) The scratch assay revealed that an conspicuous delay in the wound closure rate of TJ899/U251-vshRNA-UBE3C cells was found at 24 and 48h, compared with TJ899/U251-Mock cells(bar = 100 μm).