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. 2015 Jun 11;5:11066. doi: 10.1038/srep11066

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Identification of the peptide from the UBE3C associated protein by Mass spectra, and there are ten proteins onerlap in U251, U87 and TJ899 cells, and the overlap protein was listed in the table; (B and C) Lysates of 293T cells transfected with plasmids encoding the Flag-UBE3C or V5-ANXA7 proteins were immunoprecipitated with the indicated antibodies and analyzed by immunoblotting with anti-Flag or V5 antibodies. (D, E and F) Co-IP assays demonstrated that UBE3C forms a complex with ANXA7 in gliomas cells; (G) Localization of UBE3C (green) and ANXA7 (red) in gliomas cells by immunofluorescence (Magnification, × 1,000);.