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. 2015 Jun 11;5:11066. doi: 10.1038/srep11066

Figure 4. UBE3C promote gliomas progression by mediating ANXA7 degradation (A).

Figure 4

The UBE3C on ANXA7 ubiquitylation in vivo. V5-ANXA7 and HA-Ub were co-transfected with Flag-UBE3C into 293T cells, and lysates of 293T cells were immunoprecipitated with the indicated antibodies and analyzed by immunoblotting using the anti-Flag, V5 and HA antibody, which detected poly-ubiquitylated ANXA7. (B) ANXA7 degradation is inhibited after UBE3C interference. (C) The correlation of UBE3C and ANXA7 mRNA in gliomas tissues; (D) The expression of UBE3C and ANXA7 proteins in gliomas tissues; (E and F) The forced loss of UBE3C up-regulated the ANXA7 protein, while not the ANXA7 mRNA; (G) Expression of UBE3C and ANXA7 protein was analyzed by IHC in gliomas tissues (bar = 200μm); (H) The expression of ANXA7 were regulated in TJ899 and U251-vshUBE3C cells by RNA interference; (I) Matrigel invasion assays showed that down-regulation of ANXA7 was accompanied by a descend invasion in TJ899 and U251-vshUBE3C cells (bar = 200μm).