Figure 5. Overexpression of SUFU reduced but silencing increased allogeneic T-cell proliferation in MLR using THP-1 cells induced into APCs.

SUFU was either ectopically expressed in myeloid cell line THP-1 (A) or silenced in THP-1 cells by siRNA (B). C, SUFU overexpressed and silenced THP-1 cells were then induced into APCs and used for MLR. D, Surface expression of HLA-DR (upper) and MFI (bottom) are shown. Mock indicates THP-1 cells without genetic manipulation; SUFU⁺ indicates ectopically SUFU-expressing THP-1 cells; SUFU⁻¹ and SUFU⁻² indicate two separate siRNAs used to silence SUFU in THP-1 cells; * indicates p-value <0.05, ** indicates p-value <0.01.