Figure 2. Reprogramming timing and workload control.

A. Viral transduction in cells at different plating time points. Fibroblast cells (PCS201-012 right graph and CCD-1079 left graph) were cultured for 2 days to confluence, and one set of cells were directly transduced with GFP sendai virus, and the other set of cells were dissociated and then replated 2 hours before the transduction, and the GFP expression was analyzed 2 days after the transduction. Experiments were done in triplicate (CCD1079, p > 0.30; PCS201-012, p < 0.05) B. Comparison of reprogramming efficiency for cells with different plating timings. Fibroblast cells were cultured for 2 days until confluence, and one set of cells were directly transduced with CytoTune^TM 2.0 virus, and the other set of cells were dissociated and then replated 2 hours before the transduction, and the colonies were stained 25 days after transduction. C. Individual iPSC colony emergence after serial dilution. D. Cell survival on plates coated directly with E8 medium. E. Medium change schedule during reprogramming.