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. 2015 Jun 11;5:11319. doi: 10.1038/srep11319

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A. Flow chart for reprogramming of multiple lines. B. Typical flow cytometry profile of iPSCs. iPSCs were expanded for two passages after reprogramming and stained with Tra1–60 and NANOG antibodies with fluorescent markers. C. Average staining from 189 iPSC clones (derived from 72 cell lines). 189 iPSC clones were clonally expanded and stained, and the average NANOG/Tra1–60 positive ratios were shown D. and E. Pluripotent and differentiation gene expression profile in qPCR analysis and Fluidigm Biomarker qPCR analysis.