Table I.
Polymerase chain reaction primers for amplification of ATP2A2 from genomic DNA.
| Exon | Forward primer sequence | Reverse primer sequence | Annealing temperature (°C) |
|---|---|---|---|
| 1 | CGAGGCGGAGGCGAGGAG | GGAGCCGAAGCCCACGCG | 62.0 |
| 2 + 3 | ACCTCCCTCTTGACACATTG | GACAACTCCTAACCACACTG | 55.1 |
| 4 | CGTGCCATTTCTCTTCTAGG | CTCAACACATCAGGAAAAACAG | 55.1 |
| 5 | AGTGTCAGGCAGGTCTTTAC | AGGAAGGGAGGTGCTAAAAC | 55.1 |
| 6 | AGCCTCATTCTCTTCCTTCC | ATGGAGCGAGACTAAAGCAC | 55.1 |
| 7 | CTTGGTGTGGGTCGCAGAG | CCTTTAGAATGATAGCCAGTG | 50.3 |
| 8 | GTTGTATGGCTGGTTGCTTG | GAACAAAGAACCACGACACG | 52.0 |
| 9 | GGTTGTTTGCCTTTGTCCTAA | ATAACAAACACAAATCCCTCTT | 50.3 |
| 10 | GGCGACCATACCCTGCTC | CCCACCCCACCCTTGAAC | 55.0 |
| 11 | TCAGAGGAGGATAAAAATGGC | CTGTAAGTTTGAGGAGATAAGG | 52.0 |
| 12 + 13 | ATTGCCACCCAGTAGTATCC | GAACTGTTTGACCTTTTGCTTG | 55.1 |
| 14 | CTAGAACTTGCCACTTTTATTTA | GAGGCTACTATGTGCTTGTG | 50.0 |
| 15 | TTTCCTCCTGCTTCCCATTC | GCAATCTGGAGAGCAAACTG | 55.0 |
| 16 | TCATTTATTTTTCTGGAGGAGG | CATCTCTGTCTTTTGCTACCC | 53.8 |
| 17 | TGATCTTCGTCCTTGTGGGG | TGATAGATACCGAAACCACAG | 53.8 |
| 18 | GGGTTGGAGCCTGGACTTG | TTTTGGGAAGGGAAGAACTGT | 55.0 |
| 19 + 20 | TCCCCACCTCTCCTTGCTC | CCTCCATCACCAGCCAGTAT | 57.5 |
| 21 | GTTCCTTTTCATCTGTCGCTG | TCTTTTTCCCCAACATCAGTC | 53.8 |