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. 2015 Apr 3;12(2):1838–1844. doi: 10.3892/mmr.2015.3594

Figure 1.

Figure 1

Characterization of recombinant plasmids. (A) Electrophoretogram of CDX2 PCR products. (B) Electrophoretogram of hTERTp PCR products. (C) Restriction endonuclease enzyme digestions of pLVX-5HRE-hTERTp-CDX2-3FLAG DNA. (D) Restriction endonuclease enzyme digestions of pLEGFP-N1-5HRE-hTERTp DNA. Lanes: 1, DNA marker; 2 and 3, PCR or enzyme-digested products. (E) Schematic chart of the recombinant plasmid pLEGFP-N1-5HRE-hTERTp. (F) Schematic chart of the recombinant plasmid pLVX-5HRE-hTERTp-CDX2-3FLAG. CDX2, caudal-related homeobox; PCR, polymerase chain reaction; hTERTp, human telomerase reverse transcriptase promoter; bp, base pairs.