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. 2015 Jun 12;6:373. doi: 10.3389/fpls.2015.00373

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Copyright © 2015 Hadwiger.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Pea chromatin spreads. (A) Actinomycin D-³H was applied 1 h to pea endocarp tissue. The silver precipitates (long arrow) represent the presence of this intercalating agent in areas of dispersed pea chromatin. The short arrow indicates condensed chromatin. (B) Chromatin from pea tissue treated with actinomycin D (2 μg/ml) and pulse labeled 1 h with uridine-³H. Larger arrow indicates an aggregate of chromatin and the broad arrow indicates the presence of RNA-³H. The cytological techniques are described in Hadwiger and Adams (1978).