FIGURE 3.

Septal localization of lipid synthases in B. subtilis cells. Typical florescence images from cells harboring gfp fusions in the amyE locus cultivated in sporulation medium (DSM) up to late logarithmic growth phase are shown. Green fluorescence from the GFP-fusions was detected by using a standard GFP(R)-BP filter unit. For the name of the enzymes and their catalytic activities refer to the figure of the biosynthetic pathway (Figure 5B). Panels are from Figures 4 and 5 of Nishibori et al. (2005), © American Society for Microbiology. GFP-fusions of ClsA and PssA are located in the septal membranes even when the corresponding genes are expressed from their natural promoters (see Figure 4 of Nishibori et al., 2005).